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roche lightcycler 96 user manual

LightCycler®96は、電気機械機器です。 本機が本マニュアルおよびLightCycler® 96 User Training Guideに従って使用されない場合、 感電や身体的負傷の可能性があります。 緊急時には直ちに機器のコンセントを抜いてください。

roche lightcycler 96 user manual

翻訳 · This website uses cookies to provide you with a more responsive and personalized service. In order to proceed to login, however, you must formally accept our cookie policy. ®LightCycler 480 RNA Master Hydrolysis Probes 6 lifescience.roche.com 2. How to Use this Product y Version: 8 2.2. Protocols The following procedure is optimized for use with the ®LightCycler 480 or LightCycler® 96 System. Program the LightCycler® 480 or LightCycler® 96 Instrument before preparing the reaction mixes. 翻訳 · Roche LightCycler Whether your interest is in gene expression profiling or in detecting genetic variations, there is a member of the LightCycler® System family offering the analytical performance and throughput you need for your research. 翻訳 · Get high-quality products from Roche, the leader in molecular diagnostics, because Discovery is in our DNA. 翻訳 · The cobas ® 4800 system features state-of-the-art, fully automated sample preparation combined with real-time polymerase chain reaction (PCR) technology for amplification and detection of nucleic acid, plus easy-to-use software that seamlessly integrates both components. This combination of elements provides true walk-away automation for maximum lab efficiency. ®LightCycler 480 SYBR Green I Master 6 lifescience.roche.com 2. How to Use this Product y Version: 13 Two-step RT-PCR LightCycler® 480 SYBR Green I Master can also be used to perform two-step RT-PCR. In two-step RT-PCR, the reverse transcription of RNA into cDNA is separated from the other reaction steps and is performed outside the lifescience.roche.com For general laboratory use. R LightCycler® FastStart DNA Master HybProbe y Version: 16 Content version: December 2016 Easy-to-use hot start reaction mix for PCR using HybProbe Probes with the LightCycler® Carousel-Based System Cat. No. 03 003 248 001 1 kit 96 reactions of 20 μl final volume each Cat. No. 12 239 272 001 ... 翻訳 · LightCycler ® 96 System. ... MagNA Pure 96 System. Designed for an accelerated workflow, the MagNA Pure 96 System is optimized for high throughput nucleic acid purification ... Start confidently and increase your workflow efficiency with Roche manual and automated solutions. lifescience.roche.com For life science research only. Not for use in diagnostic procedures. R LightCycler® RNA Master SYBR Green I y Version: 13 Content version: October 2017 Easy-to-use reaction mix for one-step RT-PCR, using the LightCycler® Carousel-Based System Cat. No. 03 064 760 001 1 kit 96 reactions of 20 μl final volume each 翻訳 · The adapter plate is for use with the LightCycler ® 480 System and holds up to 12, LightCycler ® 8-Tube Strips (white). The Adapter Plate cannot be used on the LightCycler ® 96 Instrument.. Benefits. The Adapter Plate is made of blue eloxided aluminum and can be reused multiple times. 翻訳 · Specification Each lot of this kit is function tested using the MagNA Pure 96 DNA and Viral NA Large Volume Kit (Cat. No. 06 374 891 001) with the MagNA Pure 96 and LightCycler ® 480 Instrument II, using A. laidlawii at 3 and 10 cfu/mL in a 1 mL CHO cell culture with 5 × 10 6 cells/mL. In addition the kit is tested for the absence of mycoplasmas. 翻訳 · Expanding connection of up to three MagNA Pure 96 instruments fulfills high throughput requirement. Read More. FLOW PCR Set Up Instrument. The second liquid handler is also GMP manufactured by Hamilton. It prepares 96- and 384-well qPCR plates in data driven mode and allows high-speed, multi-dispensing options. Roche qPCR Instrument 翻訳 · Expanding connection of up to three MagNA Pure 96 instruments fulfills high throughput requirement. Read More. FLOW PCR Set Up Instrument. The second liquid handler is also GMP manufactured by Hamilton. It prepares 96- and 384-well qPCR plates in data driven mode and allows high-speed, multi-dispensing options. Roche qPCR Instrument 翻訳 · 05.06.2020 · User Assistance provides interactive user documentation and guidance to assist the user in his daily routine operating Roche analyzer instruments beyond traditional online help. Find information about maintenance, ... LightCycler® 96. Support & training materials. Training - LightCycler® Qualitative Analysis. 翻訳 · LightCycler 2.0 Real Time PCR System Complete w WARRANTY This is a Nice PreOwned Roche Lightcycler 2.0 System with Lightcycler Software 4.1. Comes Complete Roche LightCycler 480 II Roche Life Science 5015278001 VWR Adhesive Film for Microplates VWR 60941-070 LightCycler 480 Multiwell Plate 96, Clear Roche Life Science 5102413001 Applied Biosystems 7500 Fast Dx Real-Time PCR Instrument ThermoFisher Scientific 4406984 翻訳 · ROCHE LIGHTCYCLER 480 WELL THERMAL CYCLER SYSTEM 480/96; ROCHE LIGHTCYCLER 480 WELL THERMAL CYCLER SYSTEM 480/96. Manufacturer: Roche Model: 480 Condition: Used. See More Information. ... Use of this Web site constitutes acceptance of the LabX User Agreement. ... The RNAspin 96 Kit is designed for fast 96-well, small-scale manual isolation of total RNA from tissues or cells in the microtiter plate format using suitable vacuum manifolds (see section 6) or suitable centrifuges. This kit can also be used in a fully automated flow with vacuum on common laboratory workstations (contact Technical 翻訳 · The cobas ® 4800 system offers state-of-the-art test menu, fully automated sample preparation, real-time PCR amplification/detection and easy-to-use software for multiple sample types (the detection of Chlamydia). trachomatis (CT), Neisseria. gonorrhoeae (NG), Human Papillomavirus (HPV) and an expanding menu of assays.. It consists of the cobas x 480 instrument for the nucleic acid extraction ... 翻訳 · PCR Cycler meets the highest criteria in thermal performance. Find online auctions and classifieds ads for PCR Cyclers and more at LabX. 翻訳 · The Piko Thermal Cycler is an ideal solution for both convential and fast PCR applications. Piko Thermal Cyclers are available with two different block configurations: 24-well and 96-well. 24-well cyclers accept all standard low profile single tubes, 8-tube strips and 24-well Piko PCR Plates. The 96-well instrument utilizes 96-well Piko PCR Plates. analysis of the sorted single cells using the Roche LightCycler® Nano and LightCycler® 96. Furthermore, using data from sorted single cells, we demonstrate the ability to integrate phenotypic information obtained by flow cytometry with single cell gene expression analysis. Read Free Service Manual Cobas Integra 400 Plus Netbizlutionsday's free books as well but you must create an account before downloading anything. A free account also gives you access to email alerts in all the genres you choose. Service Manual Cobas Integra 400 Roche COBAS INTEGRA 400 plus Pdf User Manuals. View online or download Page 4/20 翻訳 · LightCycler™ 96 (Roche Diagnostics) was used in this experiment. HeLa S3 cells were incubated for 20 h after being seeded in six-well plates at 4 × 105 cells/well and treated with or without 100 nM phorbol 12-myristate 13-acetate. 翻訳 · Roche LightCycler 480 Fast setting) 10–30 sec Standard 60 sec Qiagen Rotor-Gene and Fast 10–30 sec Stratagene Mx series * 98°C is highly recommended for genomic DNA template to ensure complete denaturation. ** Shorter annealing/extension times (1–10 sec) can be used for amplicons <100 bp. Longer annealing/extension Instruction manual for THUNDERBIRD Probe One-step qRT-PCR Kit 2004 ... LightCycler® 96-3. CFX96 Touch ™ Deep Well [6] Optimization of conditions [7] Application data [8] Troubleshooting [9] Related products . ... Roche cyclers, Bio-Rad cyclers, Qiagen cyclers, etc. Not required 翻訳 · 5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl 2 , SYBR ® Green I, ROX Normalization Dyes, for 500 x 20 µl reactions 翻訳 · 200 x 20 µl reactions, 2 ml (2 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl 2, SYBR ® Green I, enhancers, stabilizers, fluorescein, and ROX normalization dyes 翻訳 · LightCycler® 96 Instrument. Public, Open and Moderated . 2 Members. MagNA Pure 24 System. Public, Open and Moderated . 2 Members. LightCycler® 480 Instrument II. ... Facilities IT - Webcast and Video Portal for Roche Group. User Guide and Content Owner Guides ... 翻訳 · Hard-Shell 96-Well Semi-Skirted PCR Plates, with either clear or white wells, are ideal for robotic handling. Low-Profile 96-Well PCR Plates Low-profile 96-Well PCR Plates in a choice of skirt formats; barcoding; clear, white, or black wells; and colored plates, including Hard-Shell ® 96-Well PCR Plates optimized for the Roche LightCycler 480 system. 96あるいは384/ラン: 測定モード: リアルタイム、オンライン (ラン中連続表示) 反応容器: 96ウェルプレート、 384ウェルプレート: 温度制御: ペルチェ素子/ Therma-Base方式による加熱/冷却: 反応液量: 96ウェル:10 ~100 μL 384ウェル:5 ~ 20 μL: ラン時間 this manual. Note: Provides important information, including information explained in further detail elsewhere in this manual. WARNING! Explains crucial information about a topic that may lead to injury to the user, instrument damage, data loss, or the prevention thereof. Touch X Touch X using your finger. LightCycler™ Detection System (Roche Applied Science) Protocol The RNAspin 96 RNA Isolation Kit was used to purify total RNA for several different applications according to the protocol illustrated in Figure 1. To reduce the risk of clogging the RNA-binding plate wells and the potential loss in sample yield and purity, the lysates were ... Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix 1 Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix MATERIALS PROVIDED Catalog #600882 (single kit), #600883 (10-pack kit) Materials Provided Quantitya,b 2× Brilliant III Ultra-Fast SYBR® Green QPCR Master Mixc, d 2 × 2 ml Reference dyed, 1 mM 100 μl a Sufficient PCR reagents are provided for four hundred, 20-μl reactions. 翻訳 · PCR 96 Well Plates, Half-Skirt PCR 96 Well Plates, Half-Skirt PCR 96 Well Plates, Half-Skirt with q-PCR Sealing Film 10 Plates/Pack, 10 Packs/Case 10 Plates/Pack, 10 Packs/Case 10 Plates and Films ... Centrifuge the 96-well reaction plate in a centrifuge with a microtiter plate rotor for 30 seconds at approximately 1000 x g (~ 3000 rpm). 6. Programming the Real-Time PCR Instrument For basic information regarding the setup and programming of the different real-time PCR instruments, please refer to the user manual of the respective instrument. 翻訳 · LightCycler® 96 Instrument. Public, Open and Moderated . 2 Members. Roche LATAM eCustomer. Public, Restricted and Moderated . 4 Members. Public. Public, Restricted and Moderated . 2 Members. Facilities IT - Webcast and Video Portal for Roche Group. User Guide and Content Owner Guides ... design guidelines refer to AllGlo™ user’s manual (www.allelogic.com). ... Roche: LIghtCycler 480, LightCycloer 2.0 31016 Fast Probe Master Mix with ROX ... Enzyme activation 96 oC 2 min 1 Denaturation Annealing & Extension 96 oC 60 oC 5 s (See Helpful Tip #3) 30 s 40 Helpful Tips: 翻訳 · During somatic cell nuclear transfer (NT), the transcriptional status of the donor cell has to be reprogrammed to reflect that of an embryo. We analysed the accuracy of this process by comparing transcript levels of four developmentally important genes (Oct4, Otx2, Ifitm3, GATA6), a gene involved in epigenetic regulation (Dnmt3a) and three housekeeping genes (β-actin, β-tubulin and GAPDH) in ...