rnascope multiplex fluorescent reagent kit v2 user manual

This user manual provides guidelines and protocols to use the RNAscope® Fluorescent Multiplex Reagent Kit (Cat. No.320850). You must use both an RNAscope® Reagent Kit User Manual and a sample preparation and pretreatment user guide to perform the entire assay. IMPORTANT! For Part 1 Sample Preparation and Pretreatment Guide, see Document No ...

rnascope multiplex fluorescent reagent kit v2 user manual

RNAscope ® Multiplex Fluorescent v2 Assay, combined with with fluorescent IHC of CD3 (Opal 690-White) in FFPE human tonsil. DAPI staining is shown in blue. For Research Use Only. Not for diagnostic use. NOTICE TO PURCHASER: PLEASE REFER TO THE RNASCOPE ® MULTIPLEX FLUORESCENT REAGENT KIT v2 USER MANUAL FOR LIMITED USE LABEL RNAscope Multiplex Fluorescent Reagent Kit v2 (Cat. No. 323100). The kit provides enough reagents to stain ~20 tissue sections, each with an area of approximately 20 mm x 20 mm (0.75in x 0.75in). The reagent kit contains pretreatment kit, detection kit, TSA buffer and wash buffer. HybEZ ™ RNAscope® Multiplex Fluorescent Reagent Kit V2 Technical Note 323120-TN Rev A Date 03012017 2 . 3. Wash slides in 1X Wash Buffer for2 MIN RTat . Repeat with fresh 1X Wash Buffer. Hybridize Amp 2 1. Remove excess liquid from slides, place in the HybEZ™ or EZ-Batch™ Slide Rack, and add4–6 drops RNAscope® Multiplex FL v2 Amp 2 to entirely ... Cy5) stocks or Opal reagent stocks using Multiplex TSA buffer provided in the RNAscope® Multiplex Fluorescent Kit v2. Recommended dilution range is 1:300–1:1500 for fluorescent IHC. 1. Add HRP-conjugated secondary antibody diluted in TBS-1% BSA to completely cover the sections. Incubate the slides for 30 MIN at RT. 2. Fluorescent Multiplex Assay (Cat. No. 320850)The required . reagent is RNAscope ® Protease III (available in RNAscope ® Protease III and Protease IV Reagents, Cat. No. or RNAscope322340 ® Universal Pretreatment Kit Cat No 322380For every chemical, ). read the Material Safety DataSheet (MSDS) and follow handling instructions. Workflow 翻訳 · RNAscope® 2.5 HD Detection Reagent – RED User Manual PART 2 Document Number 322360-USM For Part 1 Sample Preparation Pretreatment Guide for Formalin-Fixed Paraffin-Embedded (FFPE) For RNAscope® 2.5 Assay, see Document Number 322452-USM For Research Use Only (RUO). For the RNAscope ® Fluorescent Multiplex Assay PART 1 Document Number 320513-USM Compatible with the RNAscope® Fluorescent Multiplex Detection Kit User Manual Part 2, see Document Number 320293-USM. RNAscope® Fluorescent Multiplex Assay Introduction This Technical Note provides guidelines to prepare cultured adherent cells for the RNAscope® Fluorescent Multiplex Assay (Cat. No. 320850). The required reagent is RNAscope® Protease III (available in RNAscope® Protease III and Protease IV Reagents, Cat. No. 322340 or RNAscope® Universal 翻訳 · RNAscope ® 2.5 LS Assay-Brown; RNAscope ® 2.5 LS Assay-Red; RNAscope ® 2.5 LS Duplex Assay; RNAscope ® 2.5 LS Fluorescent Multiplex Assay; RNAscope ® 2.5 LSx Reagent Kit-BROWN; RNAscope ® 2.5 LSx Reagent Kit-RED; BaseScope™ LS Reagent Kit – RED the RNAscope ® Fluorescent Multiplex Assay . Introduction . This Technical Note provides guidelines to prepare Peripheral Blood Mononuclear Cells (PBMC) and non-adherent cells that can be assayed using an RNAscope ® Fluorescent Multiplex Detection Kit. The required RNAscope ® Pretreat Reagent is Protease III (available in RNAscope ® 翻訳 · SARS-CoV-2 Coronavirus Multiplex RT-qPCR Kit; SARS-CoV-2 Coronavirus Multiplex RT-qPCR Kit (CD019RT) Regulatory status: ... This product is a dual-color multiplex fluorescent probe-based Taqman® RT-qPCR assay system. ... (refer to Instrument User Manual) Take ABI7500 as an example: after the qPCR reaction, ... 翻訳 · About this guide This user manual provides guidelines and protocols for the proper preparation and pretreatment of formalin-fixed, paraffin-embedded (FFPE) tissues mounted on slides. The slides can then be assayed using an RNAscope® 2.5 Reagent Kit. RNAscope® 2.5 Reagent Kits come with a separate RNAscope® 2.5 Assay User Manual. SARS-CoV-2 Coronavirus Multiplex RT-qPCR Kit ... Principles of Testing This product is a dual-color multiplex fluorescent probe-based Taqman® RT-qPCR assay system. ... Data Analysis (refer to Instrument User Manual) Take ABI7500 as an example: after the qPCR reaction, ... Multiplex RT-qPCR Detection Kits User Manual Cat.Nos.COV00102(50 rxns×25ul) COV00103(100 rxns×25ul) ... the kit uses fluorescent labeled probes and ... Table2 Kit Contents Reagent Name Quantity (50 tests/pack) Quantity (100 tests/pack) 1.Negative control ... Reagents and materials provided in VIASURE SARS-CoV-2 Real Time PCR Detection Kit with Ref. VS-NCO236RUO and VS-NCO272RUO. For use with Qiagen/Corbett Rotor-Gene® instruments and compatible accessories with strips of 4 tubes 0.1 ml (72-Well Rotor and Locking Ring 72-Well Rotor). 5. Reagents and equipment to be supplied by the user 翻訳 · Bio-Rad offers a range of products and services for life science research and education, clinical diagnostics, biopharmaceutical processing, and food science. 翻訳 · Oestrogen signalling pathways are emerging targets for lung cancer therapy. Unravelling the contribution of oestrogens in lung cancer development is a pre-requisite to support the ViewRNA™ ISH Tissue Assay, Core Kit Each ViewRNA Tissue Assay Core Kit contains the following components, supplied in 2 boxes based on storage temperature. Reagents have a minimum shelf life of 6 months from date of receipt when stored as recommended. Please refer to kit labels for expiration dates and to the Package Insert for component 翻訳 · Antinuclear antibodies (ANA) are a group of autoantibodies produced by a person's immune system when it fails to adequately distinguish between "self" and "nonself." The ANA test detects these autoantibodies in the blood. ANA react with components of the body's own healthy cells and cause signs and symptoms such as tissue and organ inflammation, joint and muscle pain, and fatigue. HyperCap User’s Guide (v2.0) ... plus 10 µL static dead volume per reagent plate well. Manual preparation of master mixes used an additional 5% excess volume for each component to ensure adequate plating . ... – The Multiplex Sample Library Pool was normalized to 57 µL total 翻訳 · The CFX96 Touch System is a powerful, precise, and flexible real-time PCR detection system. This six-channel (five colors and one FRET channel) real-time PCR instrument combines advanced optical technology with precise temperature control to deliver sensitive, reliable detection for singlexplex or multiplex reactions. 翻訳 · Abstract. This paper presents a high-throughput reverse transcription quantitative PCR (RT-qPCR) assay for Caenorhabditis elegans that is fast, robust, and highly sensitive. This protocol obtains precise measurements of gene expression from single worms or from bulk samples. 翻訳 · Use the CFX96 optical reaction module to convert the C1000 Touch thermal cycler into a powerful six-channel real-time PCR system with precise thermal control. 翻訳 · The CFX96 Touch System is a powerful, precise, and flexible real-time PCR detection system. This six-channel (five colors and one FRET channel) real-time PCR instrument combines advanced optical technology with precise temperature control to deliver sensitive, reliable detection for singlexplex or multiplex reactions. 翻訳 · Methods: Manual and automated assays like ELISA, RIA, (electro)chemiluminescence or immunocapture methods. Resorption markers. CTX:marked circadian fluctuations, influences by food intake, stable in EDTA plasma (1 day 20°C, 3 months at -20°C). Diseases of kidney and liver interfere. IOF and IFCC recommend CTX for clinical studies. 翻訳 · Eli Lilly & Company and the National Center for Advancing ..... 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Enzyme Kinetics: Behavior and Analysis of... 翻訳 · Japan's largest platform for academic e-journals: J-STAGE is a full text database for reviewed academic papers published by Japanese societies 翻訳 · The Ad vectors for expression of AsCpf1 (Ad-AsCpf1), LbCpf1 (Ad-LbCpf1) and gRNA (Ad-asgRNA-AAVS1) were prepared by an improved in vitro ligation method. 17,20) Briefly, the fragments of expression cassettes from pHM-CBh-AsCpf1, pHM-CBh-LbCpf1, pHM-U6-asgRNA-AAVS1 and pHM-U6-lbgRNA-AAVS1 were individually integrated into the I-CeuI/PI-SceI sites of pAdHM4. 17,20) The Ad vector expressing the ... 翻訳 · Provided herein is technology relating to next-generation sequencing and particularly, but not exclusively, to methods and compositions for preparing a next-generation sequencing library comprising short overlapping DNA fragments and using the library to sequence one or more target nucleic acids. 翻訳 · The Lens serves almost all the patents and scholarly work in the world as a free, open and secure digital public good, with user privacy a paramount focus. 翻訳 · Compositions and methods for the prophylaxis and treatment of human immunodeficiency virus (HIV) infections are disclosed herein. More specifically the present invention discloses describes an autologous dendritic cell (DC) vaccine product derived by culturing a patient's monocytes with granulocyte 翻訳 · Article Congress of Clinical Chemistry and Laboratory Medicine / 10th Annual Meeting of the German Society for Clinical Chemistry and Laboratory Medicine (DGKL), Dresden, Germany, 23rd–26th October, 2013 was published on 01 Oct 2013 in the journal Clinical Chemistry and Laboratory Medicine (CCLM) (Volume 51, Issue 10). Wellcome Open Res Wellcome Open Research 2398-502X F1000 Research Limited London, UK 10.12688/wellcomeopenres.14735.2 Research Article Articles Mapping targets for small nucleolar RNAs in yeast [version 2; peer review: 5 approved] Dudnakova Tatiana Formal Analysis Investigation Methodology Visualization Writing – Original Draft Preparation Writing – Review & Editing 1 Dunn-Davies Hywel ... 翻訳 · Advances in Experimental Medicine and Biology 1008 M.R.S. Rao Editor Long Non Coding RNA Biology Advances in Experimental Medicine and Biology Volume 1008 Editorial Board: IRUN R. COHEN, The Weizmann Institute of Science, Rehovot, Israel ABEL LAJTHA, N.S. Kline Institute for Psychiatric Research, Orangeburg, NY, USA JOHN D. LAMBRIS, University of Pennsylvania, Philadelphia, PA, USA RODOLFO ... 翻訳 · Abstracts Abstracts and Case Studies From the College of American Pathologists 2015 Annual Meeting (CAP ’15) Abstract and case study poster sessions will be conducted during the 2015 College of American Pathologists Annual Meeting, which is scheduled for October 4 to 7, 2015. 翻訳 · Simplex optimization was used to derive the 12-lead ECG from just 3 basis leadvectors (I, II, V2) stored in the cardiac monitor. The CEB was computed from the derived ECG by EVA to detect AMI and then compared to both an ECG interpretive algorithm (ECGI), and to ST voltage changes (ST) consistent with … 翻訳 · Search for abbreviations and their long forms appearing in PubMed titles and abstracts. 翻訳 · User Manual REALQUALITY RS-BKV v.2.1 code RQ-S49 Kit for detection and quantification of BK virus (BKV) by Real-Time PCR RS-BKV_manual_e20130109.doc 3! 11.3.1! Thermal profile and fluorescence reading 11.3.2! Setup of samples/controls 18! 19! 3! 11.4! Preparation of the reaction mix 20! 2! KIT CONTENT 4! 3! STORAGE AND STABILITY OF REAGENTS 5 ... 翻訳 · Abstracts - Amazon AWS Nov 7, 2017 - Don't Miss the SITC Oral Poster Sessions! Beyond regular poster viewing, SITC welcomes the Oral Poster Sessions. These sessions provide ...